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Anatomy of a Comparative Gene Expression Study - http://www.cs.wustl.edu/~jbuhler//research/array/

Category: Genetics and Genomics: Cancer Genetics Protocols

Anatomy of a comparative gene expression study. Includes: Choosing Cell Populations; mRNA Extraction and Reverse Transcription; Fluorescent Labeling of cDNA's; Hybridization to a DNA Microarray; Scanning the Hybridized Array; Interpreting the Scanned Image. - [Read Anatomy of a Comparative Gene Expression Study]

Anatomy of a Comparative Gene Expression Study - http://www.cs.wustl.edu/~jbuhler//research/array/

Category: Microarray Protocols: Microarray Analysis Protocols

Information on anatomy of a comparative gene expression study. Includes: Choosing cell populations; mRNA Extraction and Reverse Transcription; Fluorescent labeling of cDNA's; Hybridization of DNA microarray; Scanning the hybridized array; Interpreting the scanned image. - [Read Anatomy of a Comparative Gene Expression Study]

Gene-specific RT-PCR NCI - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/RNA/GeneSpecificRTPCR.html

Category: RNA Protocols: RT-PCR and cDNA synthesis

Gene-specific RT-PCR. Cancer Gene Anatomy Project NCI - [Read Gene-specific RT-PCR NCI]

List of standard EM Center procedures/protocols - http://anatomy.iupui.edu/emcenter/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol for sectioning is on a separate page. 2. Standard Procedure To Section for Transmission Electron Microscopy, EM Center, Indiana University School ... List of standard EM Center procedures/protocols - [Read List of standard EM Center procedures/protocols]

Reflected Light Microscopy - http://microscopy.fsu.edu/primer/anatomy/reflected.html

Category: Microscopy Protocols

Reflected light microscopy is often referred to as incident light, epi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 microns. - [Read Reflected Light Microscopy]