User Panel

My Panel

Bookmark Science Articles

analysis Protocols

Search results for: analysis

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining and Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining; Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

Flow Cytometry Analysis Protocol - http://cbr.med.harvard.edu/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Flow Cytometry Analysis Protocol. Springer Lab - Harvard. Background on flow cytometry technique: Flow cytometry is a method which uses instrumentation scanning single cells flowing past excitation sources in a liquid medium.
- [Read Flow Cytometry Analysis Protocol]

Flow Cytometry of Apoptosis Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E663022BBACCC599F244293812ED95A&objectid=66744E3FA79C848C2D8B85BB84708AC9

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Common methods applicable to flow cytometry make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell morphology and chromatin condensation, which occur during apoptosis, can be detected by analysis with laser light beam scattering. - [Read Flow Cytometry of Apoptosis Protocol]

Fluorescent Dyes for Flow Cytometric Analysis - http://www.ebioscience.com/ebioscience/appls/Dyes.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Fluorescent dyes absorb light at certain wavelengths and in turn emit their fluorescence energy at a higher wavelength. Each dye has a distinct emission spectrum, which can be exploited for multicolor analysis. eBioscience antibodies are available conjugated to a wide variety of fluorochromes. - [Read Fluorescent Dyes for Flow Cytometric Analysis]

Fluorescent Staining of Cell Surface Antigens on Viable Cells Using Direct Immunofluorescence - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4439

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol provides a method for analysis of cell surface antigens using direct immunofluorescence, i.e., when the antibody being used is directly labeled to a fluorescent dye. After staining, the cells can be analyzed immediately by flow cytometry or fixed and stored for later analysis. - [Read Fluorescent Staining of Cell Surface Antigens on Viable Cells Using Direct Immunofluorescence]

Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol - http://www.arabidopsis.org/cshl-course/3-genetic_mapping.html

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for genetic mapping of Arabidopsis centromeres using PCR-based markers. Includes: DNA markers facilitate genome analysis; Centromere mapping using tetrad analysis; Preparation of DNA from frozen plant tissue. - [Read Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol]

Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4562

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Protein Interactions Protocols

This procedure capitalizes on the asexual/sexual reproductive cycle of yeast cells. Laboratory yeast strains can be maintained indefinitely by asexual reproduction as one of two haploid mating types, MATa or MAT{alpha}. - [Read Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array]

Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4563

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Protein Interactions Protocols

This protocol describes how to handle arrayed yeast using robotics. - [Read Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array:]

Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Amplification of ORFs - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4561

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Protein Interactions Protocols

This protocol describes the first step in constructing an array: amplification of the predicted ORFs that are to be included in the array. Gene-specific primers containing vector-specific flanking sequences that facilitate recombinational cloning are used to amplify each ORF. A secondary amplification can be used to extend the length of the homologous vector sequence flanking the ORF. - [Read Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Amplification of ORFs]

Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: S - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4564

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Protein Interactions Protocols

This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the array. - [Read Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: S]

Genome-Wide Analysis Protocol - http://www.natureprotocols.com/2006/06/29/genomewide_analysis_of_data_ge.php

Category: Microarray Protocols: Microarray Analysis Protocols

Genome-wide analysis of data generated on the Affymetrix 10K Xba 142 arrays for identification of regions with high probability to contain genes responsible for Micronodular (non-pigmented) Adrenocortical Hyperplasia. - [Read Genome-Wide Analysis Protocol]

Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4415

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

This protocol describes a rapid PCR-based method for identifying targeted ES cell colonies prior to picking. It is based on DNA analysis of a small part of colonies pooled directly from selection plates. Only positive colonies are expanded. - [Read Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol]

Glycosphingolipid Analysis Protocol - http://www.duke.edu/web/ceramide/protocols/0034.html

Category: Lipid Protocols

Protocol for glycosphingolipid analysis. - [Read Glycosphingolipid Analysis Protocol]

High Molecular Weight Yeast Liquid DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep. - [Read High Molecular Weight Yeast Liquid DNA Preparation Protocol]

How to do a Western Blot - http://www.well.ox.ac.uk/flint/Western.htm

Category: Protein Protocols: Western Blot Protocol

All the products and steps to complete a full western blotting analysis. (Jonathan Flint lab). - [Read How to do a Western Blot]

How to Isolate Nondenatured Protein Extracts from Neurospora for Western Analysis - http://www.fgsc.net/neurosporaprotocols/How%20to%20isolate%20nondenatured%20protein%20extracts%20from%20Neurospora%20for%20western%20analysis.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to isolate nondenatured protein extracts from Neurospora for western analysis. - [Read How to Isolate Nondenatured Protein Extracts from Neurospora for Western Analysis]

HPLC Separation of Digested Proteins and Preparation Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/30/pdb.prot4663

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for HPLC separation of digested proteins and preparation for matrix-assisted laser desorption/ionization analysis. - [Read HPLC Separation of Digested Proteins and Preparation Protocol]

ImageJ - Image Processing and Analysis in Java - http://rsb.info.nih.gov/ij/

Category: Molecular Imaging

ImageJ - Image Processing and Analysis in Java. Useful program for MacOs MacosX and PC. Analysis includes: Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units s - [Read ImageJ - Image Processing and Analysis in Java]

Immunofluorescent Staining for Flow Cytometry Protocols - http://www.ebioscience.com/ebioscience/appls/FCS.htm

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocols for immunofluorescent staining for flow cytometry. Includes: Protocol for Staining of Cell Suspensions of Murine Lymphoid Tissue; Protocol for Staining of Human Peripheral Blood; Fluorescent Dyes for Flow Cytometric Analysis - [Read Immunofluorescent Staining for Flow Cytometry Protocols]

Immunofluorescent Staining for the Laser Microdissection of Individual Cells Protocol - http://www.natureprotocols.com/2006/08/03/immunofluorescent_staining_for.php

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Useful techniques to circumvent disruption of tissue structure in the analysis of gene expression are LCM and LDM. While they require specialized microscopes and systems, they are similar in that freshly-cut frozen tissue sections can be microdissected using either a general histological stain (like H&E) or by staining with fluorescently conjugated antibodies. The LCM system by Arcturus involves... - [Read Immunofluorescent Staining for the Laser Microdissection of Individual Cells Protocol]

Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis - http://www.bdbiosciences.com/pharmingen/protocols/Intracellular_Cytokines.shtml

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Fluorescent anti-cytokine and monoclonal antibodies are useful for the intracellular staining and multiparameter flow cytometric analysis of individual cytokine-producing cells within mixed cell populations. BD Biosciences. - [Read Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis]

Immunohistochemistry Analysis Protocol - http://www.natureprotocols.com/2006/08/07/immunohistochemistry_analysis.php

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry analysis. - [Read Immunohistochemistry Analysis Protocol]

Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord - http://www.natureprotocols.com/2006/06/30/immunoprecipitation_ip_of_home.php

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

Protocol for the immunoprecipitation (IP) of Homer 1a, injection of virions and in situ hybridization in the spinal cord. Includes: Immunoprecipitation (IP) of Homer1a from spinal cord; Injection of virions in the parenchyma of the spinal dorsal horn in vivo; Generation of cRNA probes; Analysis of DIG-dUTP incorporation; Tissue hybridization. - [Read Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord]

In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei - http://www.plantmethods.com/content/2/1/18

Category: Plant Biology Protocols: Plant Genetics Protocols

To accurately predict the activity of a transgene it is critical to understand its location and dynamics in the 3-D interphase nucleus. Developed in situ methods to visualize transgenes (including single copy genes) & their transcripts during interphase from different tissues & plant species. These techniques reduce the time necessary for characterization of transgene integration by eliminating the need for time-consuming segregation analysis and extend characterization to the interphase nucleus - [Read In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei]

Articles (1-8 of 8)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Toluidine Blue Stain

Toluidine Blue staining method and protocol for cell or tissue histology.

Thionin Stain

Thionin staining method and protocol for cell or tissue histology.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

[1-8]

Items 76-100 out of 242 displayed.