User Panel

My Panel

Bookmark Science Articles

analysis Protocols

Search results for: analysis

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Analysis of protein phosphorylation: methods and strategies PDF - http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-313X.2005.02613.x

Category: Protein Protocols: Protein Phosphorylation Protocols

Paper describing methods for monitoring kinase activity, investigating kinaseâ€“substrate specificity, examining phosphorylation in planta and the determination of phosphorylation sites in a protein. In addition, strategic considerations for experimental design and variables will be discussed. Scott C. Peck, the Plant Journal. - [Read Analysis of protein phosphorylation: methods and strategies PDF]

Analysis of Proteins by Immunoprecipitation - http://dps.ufl.edu/hansen/protocols/imp98.prt.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody. Immunoprecipitation protocols / methodology and technical background information. P.J. Ha - [Read Analysis of Proteins by Immunoprecipitation]

Analysis of Proteins using Small Format 2D Gel Electrophoresis - http://www.abdn.ac.uk/~mmb023/protocol.htm

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Analysis of Proteins using Small Format 2D Gel Electrophoresis. Cash Lab - [Read Analysis of Proteins using Small Format 2D Gel Electrophoresis]

Analysis of Recombinant Bacteriophage M13 Clones Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3996

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

A rapid method to analyze the size of the single-stranded DNA of M13 recombinants. - [Read Analysis of Recombinant Bacteriophage M13 Clones Protocol]

Analysis of the Plant Epidermis Experiments - http://www.arabidopsis.org/cshl-course/6-epidermis.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Experiments included for analysis of plant epidermis: Steroid induction of gene expression in Arabidopsis; Replica Molds and Casts of the plant epidermis. - [Read Analysis of the Plant Epidermis Experiments]

Annexin V Staining Flow Cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/page1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Annexin V Staining Flow Cytometry Protocol - a general protocol for staining cell for cytometry analysis. BD PharMingen. - [Read Annexin V Staining Flow Cytometry Protocol]

Antibody Array Production and Analysis - http://www.stanford.edu/group/robinsonlab/protocols/protocols_tools.htm

Category: Antibody Microarray Protocols

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

Assembling Aggregates between Diploid and Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4425

Category: Mouse Protocols: Assembling Chimeras Mouse Protocols

Protocol describes a method for producing diploid embryo-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for diploid embryo-diploid embryo aggregation. The resulting chimeras are useful for phenotypic analysis when an induced mutation has an extraembryonic phenotype. - [Read Assembling Aggregates between Diploid and Tetraploid Embryos Protocol]

Assessment of Magnaporthe grisea Mating Type by Spore PCR - http://www.fgsc.net/fgn42/xu.html

Category: Fungi Protocols: Fungi Genetics Protocols

PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA. - [Read Assessment of Magnaporthe grisea Mating Type by Spore PCR]

Baculovirus Analysis of Nonsecreted Protein Expression Protocol - http://www.ebioscience.com/ebioscience/appls/BV.htm#B

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirus analysis of nonsecreted protein expression. - [Read Baculovirus Analysis of Nonsecreted Protein Expression Protocol]

Baculovirus Protein Expression Protocols - http://www.ebioscience.com/ebioscience/appls/BV.htm

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Baculovirus protein expression protocols. Includes protocols: Cotransfection Protocol; Analysis of nonsecreted protein expression; Growing and Freezing Insect Cells. - [Read Baculovirus Protein Expression Protocols]

Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscrip - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4435

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscription Required). Biochemical Analysis of Cell Death Using Colorimetric Quantification of Caspase Activation Shailaja Kasibhatla et al., - [Read Biochemical Analysis Of Cell Death Using Colorimetric Quantification Of Caspase Activation (Subscrip]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

Bisulfite-PCR for Restriction Analysis and/or Sequencing - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing]

Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for bisulfite-PCR for restriction analysis and/or sequencing. Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol]

Blood Collection by Tail Bleeding Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4388

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for collection of blood from the tail of a mouse. The blood can then be used for analysis. - [Read Blood Collection by Tail Bleeding Protocol]

Blue-Native PAGE in Plants: A Tool in Analysis of Protein-Protein Interactions - http://www.plantmethods.com/content/1/1/11

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science. - [Read Blue-Native PAGE in Plants: A Tool in Analysis of Protein-Protein Interactions]

Cajal Body Isolation Protocol - http://www.lamondlab.com/f7cbprotocol.htm

Category: Cell Organelle Protocols: Nucleus Protocols

Cajal Body Isolation Protocol. Protocol includes: Sonication, Removal Nucleoi, Gradient One, Gradient two, Concentration and final enrichment of cajal bodies. Also includes: Making 2.55M sucrose stock and Analysis of the enriched Cajal body fraction. - [Read Cajal Body Isolation Protocol]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for calcium flux analysis. Includes: Reagent Preparation; Sample Preparation; Cell Surface Staining; Instrument Set up; Optimizing the Instrument Settings; Recording Data to Apply Compensation; Recording Experimental Data. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for calcium flux analysis for flow cytometry. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

cDNA / AFLP - http://www.dpw.wau.nl/pv/aflp/cDNA-AFLP%20Protocol.htm

Category: Genetics and Genomics: Cancer Genetics Protocols

CDNA/AFLP. A tool for transcriptome analysis. - [Read cDNA / AFLP]

Cell Cycle Analysis - Propidium Iodide - http://science.cancerresearchuk.org/sci/facs/facs_major_apps/cell_cycle_analysis/propidium_iodide/?version=1

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Information on how to quantitatively assess DNA content. Includes: Propidium iodide staining protocol and staining of DNA by Mithramicin Protocol - [Read Cell Cycle Analysis - Propidium Iodide]

Cell Cycle Analysis - Propidium Iodide - http://science.cancerresearchuk.org/sci/facs/facs_major_apps/cell_cycle_analysis/propidium_iodide/?version=1

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Information on propidium iodide for cell cycle analysis. - [Read Cell Cycle Analysis - Propidium Iodide]

Cell Cycle Protocol using Flow Cytometry - http://www.people.cornell.edu/pages/jls269/protocols/cellcycleprotocols.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Cell Cycle Protocol using Flow Cytometry. Cell cycle Analysis by Flow Cytometry. DNA content Analysis on the FACSAria. Analyzing Cell cycle FCM data. Biomedical Sciences Flow Cytometry Core Laboratory. Dr.Smith Cornell. - [Read Cell Cycle Protocol using Flow Cytometry]

Articles (1-8 of 8)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Toluidine Blue Stain

Toluidine Blue staining method and protocol for cell or tissue histology.

Thionin Stain

Thionin staining method and protocol for cell or tissue histology.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

[1-8]

Items 26-50 out of 242 displayed.