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Co-immunoprecipitation Analysis of Protein-Protein Interactions - http://www.uphs.upenn.edu/mcrc/morrisey/coip.htm

Category: Protein Protocols: Co-immunoprecipitation

Co-immunoprecipitation Analysis of Protein-Protein Interactions. Morrisey Lab - [Read Co-immunoprecipitation Analysis of Protein-Protein Interactions]

Analysis of Proteins by Immunoprecipitation - http://dps.ufl.edu/hansen/protocols/imp98.prt.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody. Immunoprecipitation protocols / methodology and technical background information. P.J. Ha - [Read Analysis of Proteins by Immunoprecipitation]

How to do a Western Blot - http://www.well.ox.ac.uk/flint/Western.htm

Category: Protein Protocols: Western Blot Protocol

All the products and steps to complete a full western blotting analysis. (Jonathan Flint lab). - [Read How to do a Western Blot]

Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis - http://www.ambl.msl.ubc.ca/exp2.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Very detailed class protocol for Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis. David Ng, UBC. - [Read Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

UV Spectrophotometric Analysis of DNA and RNA - http://www.people.hofstra.edu/faculty/Beverly_Clendening/Adv_Molecular_Biology/Protocols/UV_Spec_Analysis_RNA&DNA.htm

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Determining the concentration of a RNA or DNA sample by using UV spectrophotometry. Hofstra Univ. Beverly Clendening. - [Read UV Spectrophotometric Analysis of DNA and RNA]

Plant Protein Isolation - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/ProteinIsolation.pdf

Category: Protein Protocols: Protein Extraction From Tissue

Plant Protein Isolation - Rapid isolation of protein for SDS-PAGE analysis. (Essentially the same protocol as that described for GUS Assays). Stockinger Lab - [Read Plant Protein Isolation]

A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE) - http://www.fgsc.net/fgn45/45meyer.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Bioch - [Read A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE)]

Mass spectrometry analysis and identification of proteins - http://www.healthsystem.virginia.edu/internet/biomolec/mass_spectrometry.cfm

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]

Analysis of protein phosphorylation: methods and strategies PDF - http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-313X.2005.02613.x

Category: Protein Protocols: Protein Phosphorylation Protocols

Paper describing methods for monitoring kinase activity, investigating kinaseâ€“substrate specificity, examining phosphorylation in planta and the determination of phosphorylation sites in a protein. In addition, strategic considerations for experimental design and variables will be discussed. Scott C. Peck, the Plant Journal. - [Read Analysis of protein phosphorylation: methods and strategies PDF]

Analysis of methylation using bisulphite sequencing - http://www.methods.info/Methods/DNA_methylation/Bisulphite_sequencing.html

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated cytosines remain unchanged. Dr. A. Gratchev Methods.info - [Read Analysis of methylation using bisulphite sequencing]

Media and Culture of Yeast Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E5192D47CE9B0776DB7D136C34ED148A&objectid=6673ABA6C2DB2E4E7564EDFA26589256

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast. - [Read Media and Culture of Yeast Protocol]

Protein Isolation and Analysis From Plants - http://csm.jmu.edu/biology/courses/bio480_580/mblab/protein_isolation.htm

Category: Protein Protocols: Protein Extraction From Tissue

Protein Isolation and Analysis From Plants. T. Rife. Department of Biology, James Madison University. Harrisonburg, VA - [Read Protein Isolation and Analysis From Plants]

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis - http://www.jhc.org/cgi/content/full/46/3/397

Category: Protein Protocols: Protein Extraction From Tissue

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections. Ikedaa et al, 1998 JHC. - [Read Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis]

Cell Cycle Protocol using Flow Cytometry - http://www.people.cornell.edu/pages/jls269/protocols/cellcycleprotocols.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Cell Cycle Protocol using Flow Cytometry. Cell cycle Analysis by Flow Cytometry. DNA content Analysis on the FACSAria. Analyzing Cell cycle FCM data. Biomedical Sciences Flow Cytometry Core Laboratory. Dr.Smith Cornell. - [Read Cell Cycle Protocol using Flow Cytometry]

Cell Cycle Analysis - Propidium Iodide - http://science.cancerresearchuk.org/sci/facs/facs_major_apps/cell_cycle_analysis/propidium_iodide/?version=1

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Information on how to quantitatively assess DNA content. Includes: Propidium iodide staining protocol and staining of DNA by Mithramicin Protocol - [Read Cell Cycle Analysis - Propidium Iodide]

Real-Time or Kinetic PCR - http://dna-9.int-med.uiowa.edu/realtime.htm

Category: Real Time PCR

Real-Time Chemistry, Instrumentation, Real-Time PCR Quantitation, Quantitative Primer and Probe Design, Thermal Cycling Parameters, Sample Preparation, and Real-time Data Analysis. - [Read Real-Time or Kinetic PCR]

Preparation of Protein Extracts for 2D Gel-analysis - http://www.riedlab.nci.nih.gov/publications/2425.1533_2D_Gel_Protein_Ext.pdf

Category: Protein Protocols: Protein Extraction From Cells

Preparation of Protein Extracts for 2D Gel-analysis. Thomas Ried Lab NCI. - [Read Preparation of Protein Extracts for 2D Gel-analysis]

Millipore's ZipTip Protocol - http://www.millipore.com/publications.nsf/docs/tn072

Category: Mass Spectrometry Protocols

Millipore's ZipTip Protocol. Intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. Millipore. - [Read Millipore's ZipTip Protocol]

A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

Methods for Plant RNA Isolation - http://wheat.pw.usda.gov/~lazo/methods/lazo/met6.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

AMES/Chloroform extraction to extract total RNA from potato leaves for viroid analysis. - [Read Methods for Plant RNA Isolation]

Proteomic protocols and Peptide Extraction - http://www.curie.fr/recherche/themes/equipe-protocoles.cfm/id_equipe/310/lang/_gb.htm

Category: Mass Spectrometry Protocols

Colloidal coomassie blue staining, Destaining, Reduction/Alkylation, Peptide extraction For MALDI-MS analysis and For MALDI-MS analysis protocols and methods. Institut Curie Paris. - [Read Proteomic protocols and Peptide Extraction]

FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING - http://www.vetmed.auburn.edu/index.pl/cell_cycle/dna_content

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Analysis of Cell Cycle Based on DNA Content. FIXATION PROTOCOL FOR FLOW CYTOMETRIC ... Liberate cells from tissue culture flask by trypsin digestion and ... FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING. Auburn University, College of Veterinary Medicine. You. - [Read FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING]

Protocols for Mass Spectrometry Analysis Preparation - http://www.st-andrews.ac.uk/~bmsmspf/protocols.htm#manual

Category: Mass Spectrometry Protocols

Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY - [Read Protocols for Mass Spectrometry Analysis Preparation]

Articles (1-8 of 8)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Toluidine Blue Stain

Toluidine Blue staining method and protocol for cell or tissue histology.

Thionin Stain

Thionin staining method and protocol for cell or tissue histology.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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