ambion Protocols

Category: RNA Protocols: RT-PCR and cDNA synthesis

Avoiding DNA Contamination in RT-PCR Ambion - [Read Avoiding DNA Contamination in RT-PCR Ambion]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

The ability to synthesize RNA in the lab is critical to many techniques.Radiolabeled and nonisotopically labeled RNA probes, generated in small scale transcription reactions can be used in blot hybridizations and nuclease protection assays. This article includes information on: Requirements For Transcription, RNA Phage Polymerases, Template Options: Plasmids, PCR Products, Oligonuclotides and cDNA, Sense or Antisense, Conventional Or Large Scale Synthesis, Products for In Vitro Transcription. - [Read Basic Information on In Vitro Transcription]

Category: Molecular Biology Protocols: Agarose Gel Preparation

The quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel. - [Read Denaturing Agarose Gel Electrophoresis of RNA]

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Protocol for denaturing agarose gel electrophoresis of RNA. Includes:  Prepare the gel; Prepare the RNA sample; Electrophoresis. - [Read Denaturing Agarose Gel Electrophoresis of RNA Protocol]

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Three Ambion kits were used to quantitate specific miRNAs and to detect differential miRNA expression in various mouse brain regions and cell types isolated by laser capture microdissection (LCM). These techniques can be applied to studying miRNA in other species, tissues, and cell types. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR. - [Read Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples]

Category: RNAi Technology Protocols

Information on how detect and quantitate MicroRNA in laser capture microdissection samples. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR; Detection of miRNA in Microdissected Tissue from Mouse Brain by qRT-PCR; Differential Expression of MicroRNA in Whole Brain Tissue Compared to a More Homogeneous Population of Cells. - [Read Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples]

Category: Real Time PCR

Real Time PCR the Basics Ambion - [Read Real Time PCR the Basics Ambion]

Category: siRNA and RNAi Protocols

RNAi Interference Resources - Ambion. Introduction, product database for siRNA and RNAi. Ambion. - [Read RNAi Interference Resources - Ambion]