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Alternate Sporulation Method for BY4743 Protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/spo_riles

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for an alternative method for sporulation for BY4743. - [Read Alternate Sporulation Method for BY4743 Protocol]

Isolation of Mouse Intrahepatic Lymphocytes Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66370706B026EE3B53EB4B32AD4A09&objectid=6674D486BDDA02EC25183A2F0D9BED25

Category: Mouse Protocols

Provides two protocols that may be used to isolate IHL. One can be used to isolate IHL from multiple livers in parallel, whereas the more elaborate alternate protocol yields more cells per liver but is more appropriately used to recover the IHL from a single liver. - [Read Isolation of Mouse Intrahepatic Lymphocytes Protocol]

Measurement of Intracellular Ions by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C4C9AC9935B31A1C2087E2276A3&objectid=6674EF32EE59A37C48B4A267D733CAE2

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]