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End Labeling Protruding 3' Termini of Double-stranded DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3853

Category: Nucleic Acid Modification Protocols: Nucleic Acid Radiolabeling Protocols

Protocol for end labeling protruding 3' termini of double-stranded DNA with [{alpha}-32P]Cordycepin 5'-Triphosphate or [{alpha}-32P]dideoxyATP. - [Read End Labeling Protruding 3' Termini of Double-stranded DNA Protocol]

Establishment of Stable Transfectant of CHO Lec Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_CHOlec.html

Category: Transfection Protocols: Stable Transfection Protocols

CHO Lec3.2.8.1 cells have 4 independent mutations in the N and O glycosylation pathways. When cultured with alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin, glycoproteins produced in CHO Lec3.2.8.1 cells are completely susceptible to Endo H digestion. Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site which is ideal for maintenance of protein solubility and special carb-protein interactions, such as between the first N-acetyl glucosamine residue and tryp. - [Read Establishment of Stable Transfectant of CHO Lec Cells Protocol]

Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4562

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Protein Interactions Protocols

This procedure capitalizes on the asexual/sexual reproductive cycle of yeast cells. Laboratory yeast strains can be maintained indefinitely by asexual reproduction as one of two haploid mating types, MATa or MAT{alpha}. - [Read Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array]

In situ hybridisation to alpha satellite sequences (chromosome specific) - http://www.methodbook.net/probes/insitu.html

Category: Cytogenetics Protocols

In situ hybridisation to alpha satellite sequences (chromosome specific). Method book .net - [Read In situ hybridisation to alpha satellite sequences (chromosome specific)]

In Situ Hybridisation to Alpha Satellite Sequences (Chromosome Specific) Protocol - http://www.methodbook.net/probes/insitu.html

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

In situ hybridisation to alpha satellite sequences (chromosome specific) protocol. - [Read In Situ Hybridisation to Alpha Satellite Sequences (Chromosome Specific) Protocol]

In Situ Hybridisation to Alpha Satellite Sequences Protocol - http://www.methodbook.net/probes/insitu.html

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for in situ hybridisation to alpha satellite sequences (chromosome specific). - [Read In Situ Hybridisation to Alpha Satellite Sequences Protocol]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4173

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

It is sometimes desirable to have an entire population of yeast cells that is growing synchronously or is arrested at a unique point in the cell cycle. - [Read Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low Cell Concentration Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4171

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low pH Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4172

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3848

Category: Nucleic Acid Modification Protocols: Nucleic Acid Radiolabeling Protocols

In this protocol, double-stranded DNA probes, labeled in each strand, are produced in conventional PCRs containing equal concentrations of two primers, a double-stranded DNA template, three unlabeled dNTPs at concentrations exceeding the Km, and one [{alpha}-32P]dNTP at a concentration at or slightly above the Km (2-3 µm) for a thermostable DNA polymerase such as Taq. - [Read Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol]

Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3848

Category: PCR Protocols

Protocol describes how double-stranded DNA probes, labeled in each strand, are produced in conventional PCRs containing equal concentrations of two primers, a double-stranded DNA template, three unlabeled dNTPs at concentrations exceeding the Km, and one [{alpha}-32P]dNTP at a concentration at or slightly above the Km (2-3 µm) for a thermostable DNA polymerase such as Taq. - [Read Radiolabeling of DNA Probes by the Polymerase Chain Reaction Protocol]

Relative RT-PCR: Determining the Linear Range of Amplification and Optimizing the Primers:Competimer - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4109

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

In the first part of this protocol, the linear range of amplification is determined by carrying out 10 identical PCRs in the presence of [{alpha}-32P]dCTP and stopping one reaction after every two cycles. Amplification products are quantified on a denaturing polyacrylamide gel and the results plotted on a graph (counts per minute vs. cycle number). Total RNA is used as an internal control. - [Read Relative RT-PCR: Determining the Linear Range of Amplification and Optimizing the Primers:Competimer]

Sporulation and Dissection of Yeast a/alpha Diploids Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_sporulation.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for sporulation and dissection of yeast a/alpha diploids. - [Read Sporulation and Dissection of Yeast a/alpha Diploids Protocol]

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Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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