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Advanced Methodologies in Flow Cytometry - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/index1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Advanced Methodologies in Flow Cytometry. Andrea Cossarizza et al.. Materials from a workshop held March, 1997 in Modena, Italy. - [Read Advanced Methodologies in Flow Cytometry]

Detection of an advanced glycosylation product bound to protein in situ. PDF - http://www.jbc.org/cgi/reprint/260/13/7970.pdf

Category: Molecular Biology Protocols: Carbohydrate Protocols

Detection of an advanced glycosylation product bound to protein in situ. PDF . Chang JC, Ulrich PC, Bucala R, Cerami A. - [Read Detection of an advanced glycosylation product bound to protein in situ. PDF]

Light Microscopy Techniques - http://www.hei.org/research/aemi/clm.htm

Category: Molecular Imaging: Light Microscopy

Ahmanson Center for Advanced Electron Microscopy and Imaging. Use of Semi-Thin Cryosections for Light Microscopy, Immunolabeling of Cryosections on Glass Slides, Problems with Autofluorescence. House Ear Institute - [Read Light Microscopy Techniques]

Methods for Maintaining Insect Cell Cultures - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355909

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Insect cell cultures are now commonly used in insect physiology, developmental biology, pathology, and molecular biology. As the field has advanced from methods development to a standard procedure, so has the diversity of scientists using the technique. This paper describes methods that are effective for maintaining various insect cell lines. The procedures are differentiated between loosely or non-attached cell strains, attached cell strains, and strongly adherent cell strains. - [Read Methods for Maintaining Insect Cell Cultures]

Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4524

Category: Drosophila Protocols: Drosophila Staining Protocols

Early embryos (0-17 hours or until cuticle formation) are treated with a mixture of organic solvents, formaldehyde, and alcohols, as described here. The cuticles of late-stage embryos are usually opened by sonication. Tissues from more advanced stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination - [Read Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol]

Slide Preparation for Manual Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepManualMicrodissection.html

Category: Primary Cell Isolation and Culture Protocols

Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized. - [Read Slide Preparation for Manual Microdissection Protocol]