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ABC Monoclonal Antibody Staining of Dissected Larval and Adult CNS Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Staining_ItoKei.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for ABC monoclonal antibody staining of dissected larval and adult CNS. - [Read ABC Monoclonal Antibody Staining of Dissected Larval and Adult CNS Protocol]

Anti-Nogo-A Treatment Protocol - http://www.natureprotocols.com/2006/07/21/antinogoa_treatment_enhances_c.php

Category: Neuroscience Protocols

After section of the corticospinal (CS) tract in adult rats, neutralizing Nogo-A with monoclonal antibodies leads to enhancement of axonal re-growth and compensatory sprouting, accompanied by increased motor recovery. The neutralization of Nogo-A represents a promising approach for therapy after lesion if its enhancement of functional recovery can be transposed to primates. - [Read Anti-Nogo-A Treatment Protocol]

Culturing Schwann Cells from Adult Peripheral Nerve Protocol - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Rat Neuronal Cell Culture Protocols

Protocol for culturing schwann cells from adult peripheral nerve. - [Read Culturing Schwann Cells from Adult Peripheral Nerve Protocol]

Fixing Caenorhabditis elegans in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4522

Category: C. elegans Protocols: C. elegans Fixing Protocols

Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms. - [Read Fixing Caenorhabditis elegans in Paraformaldehyde Protocol]

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/14/pdb.prot4779

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

This protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues. - [Read Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol]

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/14/pdb.prot4779

Category: Laboratory Model Organisms Protocols

Protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues. - [Read Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol]

Isolation of Adult Mouse Cardiac Myocytes from One Heart Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000125.pdf?pid=PP00000125

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This procedure describes the isolation and culture of adult mouse cardiac myocytes from one heart. The isolation routinely yields approximately 1 million rodshaped myocytes per heart. - [Read Isolation of Adult Mouse Cardiac Myocytes from One Heart Protocol]

Isolation of Adult Mouse Cardiac Myocytes from One Heart Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000125.pdf?pid=PP00000125

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol describes the isolation and culture of adult mouse cardiac myocytes from one heart. The isolation routinely yields approximately 1 million rodshaped myocytes per heart. - [Read Isolation of Adult Mouse Cardiac Myocytes from One Heart Protocol]

Isolation of Adult Mouse Cardiac Myocytes from Two or More Hearts Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000126.pdf?pid=PP00000126

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This procedure describes the isolation and culture of adult mouse cardiac myocytes from two or more hearts. Includes modifications for the digestion of two or more hearts in the same procedure and subsequent pooling of myocytes derived from the multiple hearts. The isolation procedure is performed by one or more technicians and routinely yields approximately 1 million rod-shaped myocytes per heart. - [Read Isolation of Adult Mouse Cardiac Myocytes from Two or More Hearts Protocol]

Isolation of Live Bacteria from Adult Insects Protocol - http://www.natureprotocols.com/2006/06/23/isolation_of_live_bacteria_fro.php

Category: Bacterial Protocols

Protocol was developed to isolate Wolbachia from adult Drosophila, but it can be adapted for other insects. In some insects leg removal prior to isolation facilitates hemolymph extrusion. - [Read Isolation of Live Bacteria from Adult Insects Protocol]

Preparation of Myocyte RNA for Microarray Analysis Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000133.pdf?pid=PP00000133

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol permits the isolation of at least 3 µg of total RNA from approximately 150,000 cultured cardiac myocytes from adult mice. Includes: Treatment of Samples and Termination of Reactions; Isolation of RNA; Use of Environmental Chamber. - [Read Preparation of Myocyte RNA for Microarray Analysis Protocol]

Successfully culturing Schwann cells from adult peripheral nerve - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience: Neuron Cell Culture Protocols

Preparation of cultures, Ansselin et al., 1998. Acta Chururgica Austriaca 30. - [Read Successfully culturing Schwann cells from adult peripheral nerve]

Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol - http://www.natureprotocols.com/2006/11/17/thymosin_4_t4induced_outgrowth.php

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for thymosin β4 (Tβ4)-induced outgrowth and differentiation of vasculogenic precursor cells from adult epicardium. Includes: Preparation of EPDCs; Characterisation of EPDC Phenotypes by Immunofluorescence. - [Read Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol]

Transplantation of Tissues Under the Kidney Capsule Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4382

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for transplanting tissue under the kidney capsule. The in vivo developmental potential of transplanted adult or embryonic tissues can then be determined. - [Read Transplantation of Tissues Under the Kidney Capsule Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for visualizing miRNAs in adult skeletal muscle by hybridization in situ. Includes: Visualizing miRNAs in adult skeletal muscle by hybridization in situ; Probe preparation; Tissue preparation; Fixation; Deproteinization with Proteinase K; Acetylation of the tissue; Dehydration; Hybridization step; Post-Hybridization Washes (Step 1-6 should be performed on shaker); Signal Detection (Steps should be performed on a shaker); Post-Detection Washes. - [Read Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

X-Gal Staining of Drosophila Adult Head Whole Mounts Protocol - http://vosshall.rockefeller.edu/protocols/X-galWholeMount.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of Drosophila adult head whole mounts. - [Read X-Gal Staining of Drosophila Adult Head Whole Mounts Protocol]