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How to Activate Ascospores and Optimize the Recovery of Progeny - http://www.fgsc.net/neurosporaprotocols/How%20to%20activate%20ascospores%20and%20optimize%20the%20recovery%20of%20progeny.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to activate ascospores and optimize the recovery of progeny. - [Read How to Activate Ascospores and Optimize the Recovery of Progeny]

Method to Restart Stuck Fermentation - http://www.vinquiry.com/pdf/METHODTORESTARTSTUCKFERMENTATION2003.pdf

Category: Bacterial Cell Culture Protocols: Fermentation Equipment

Method to restart stuck fermentation. Includes: Select and rehydrate active dry wine yeast; activate the rehydrated yeast with nutrients and sugar; start the fermentation and add the stock wine in batches. - [Read Method to Restart Stuck Fermentation]

Raf-1 Kinase Assay - http://www.bio.com/protocolstools/protocol.jhtml?id=p1980

Category: Signalling Signal Transduction Protocols: MAPK Protocols

This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Protocol includes information about: Harvest, Immunoprecipitation and Kinase Reaction. Also includes following solutions: Laemmli Sample Buffer (1X), ATP Mix, Kinase Buffer (10X), Lysis Buffer. Helpful protocol hints are also included. - [Read Raf-1 Kinase Assay]

Articles (1-3 of 3)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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