actin Protocols

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Actin Binding & Bundling Sedimentation Assays - http://www2.bc.edu/~burgesda/protocols/Actin_Binding.html

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol includes: Binding assays for tropomyosin, villin or caldesmon and Bundling Assays - [Read Actin Binding & Bundling Sedimentation Assays]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol for actin capture assay. Includes: Actin Capture Assay Reagants. - [Read Actin Capture Assay Protocol]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Yeast Protocols

Protocol for actin capture assay. - [Read Actin Capture Assay Protocol]

Actin Staining in Fixed Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4168

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Phalloidin binds specifically to F-actin, and fluorescent-tagged phalloidin stains the actin skeleton in cells in a manner that is very close to the staining pattern seen using anti-actin antibody. - [Read Actin Staining in Fixed Yeast Cells Protocol]

Electron Microscopy of the Cytoskeleton of Cultured Cells - http://www.borisylab.northwestern.edu/pages/protocols/electmicrosc.html

Category: Microscopy Protocols: Electron Microscopy Protocols

Article describe the preparation of cells for correlative electron microscopy after live light microscopic observation of fluorescently labeled cytoskeletal proteins microinjected into the same cells. Since identification of cytoskeletal elements in electron microscopic preparations is an essential part of any correlative study, procedures for immunogold labeling of cytoskeletal components and for myosin S1 decoration of actin filaments are also described. - [Read Electron Microscopy of the Cytoskeleton of Cultured Cells]

Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol - http://www.cshprotocols.org/cgi/content/extract/2007/3/pdb.ip28

Category: Cytoskeleton Protocols: Actin Myosin Protocols

The same GFP-tagged actin construct used in cell transfection experiments has been used to produce transgenic mice. Transgenic animals allow the imaging of brain tissue in the intact animal, as acutely cut slices or as organotypic slice cultures. They also serve as a source of cells for imaging neurons at high resolution in dispersed low-density cell culture. In contrast to cells transfected in culture, where the level of actin-GFP expression in neurons varies considerably, transgenic mice... - [Read Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol]

In Vivo Actin Polymerization (cAMP) Protocol - http://www.hopkinsmedicine.org/cellbio/devreotes/invivopoly.htm

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol for the in vivo actin polymerization. - [Read In Vivo Actin Polymerization (cAMP) Protocol]

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN - http://ylwang.umassmed.edu/protocol/cfs/dbp.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN. Indirect and direct methods. Yu li Wang labs. - [Read STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol]

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Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.