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A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb16/16183-2.pdf

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The protocol includes: organelle isolation, deoxyribonuclease treatment, lysis, deproteinisation and a final DNA purification with sodium dodecyl sulphate and potassium acetate. The organelle DNA yield is 5–10 micrograms per gram of tissue and the DNA is fully restrictable. The technique is inexpensive and appropriate for the isolation of multiple samples of organelle DNA from a small amount of tissue. - [Read A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower]

Concentration of Oligo DNA by Ethanol Precipitation Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html

Category: Oligonucleotide Protocols: Oligonucleotide Purification Protocols

Concentration of DNA by Ethanol Precipitation Protocol. Adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma. Usually 2.5 - 3 volumes of ethanol and/or acetate solution is added to the DNA in a microcentrifuge tube. This is then put into an ice-water bath for at least 10 minutes. The precipitation is performed by incubation at -20C overnight. - [Read Concentration of Oligo DNA by Ethanol Precipitation Protocol]

Electron Microscope Lab protocols - http://biology.berkeley.edu/EML/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Generic processing protocol ï¿½ PLT/ LR Gold ï¿½ SEM Generic Processing protocol ï¿½ SEM Microwave tissue processing protocol ï¿½ Uranyl acetate and Lead citrate ...Electron Microscope Lab protocols at UC Berkeley - [Read Electron Microscope Lab protocols]

In Vitro Transcription With Yeast Nuclear Extract - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_trans.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with yeast nuclear extract. Includes recipes for: 5x Acetate transcription buffer; Creatine phospho kinase; Phospho creatine; Stop mix; HA + 0.1 M potassium acetate; 5x glutamate transcription buffer (5 ml). Includes also protocol for Primer Extension Assay. - [Read In Vitro Transcription With Yeast Nuclear Extract]

Lithium Acetate Transformation of Yeast Protocol - http://www.genomics.princeton.edu/dunham/yeasttransformation.htm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for lithium acetate transformation of yeast. - [Read Lithium Acetate Transformation of Yeast Protocol]

LS-L929 Cytotoxcitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/38.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

This simple cell culture-based cytotoxicity test (in which cell viability is determined by uptake of the dyes ethidium bromide and fluorescein acetate) has been developed as a general test for acute toxicity. - [Read LS-L929 Cytotoxcitiy Test]

Monosaccharide Analysis by Methanolysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4247

Category: Chromatography Protocols: Gas Chromatography Protocols

Protocol describes monosaccharide analysis by methanolysis. The method gives more complex chromatograms than the corresponding alditol acetate method, because up to four methyl glycosides can be produced from each monosaccharide. However, the method is easier to perform. - [Read Monosaccharide Analysis by Methanolysis Protocol]

Protein extraction from whole tissues for isoelectric focusing - http://proteomics.missouri.edu/protocols/proteinExtraction.html

Category: Protein Protocols: Protein Extraction From Tissue

Protein extraction from whole tissues for isoelectric focusing. University of Missouri - Columbia Proteomics Center. SDS extraction followed by acetone precipitation. Also has Phenol extraction followed by methanolic ammonium acetate precipitation. - [Read Protein extraction from whole tissues for isoelectric focusing]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

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