Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs Protocol Protocol describes the growth and concentration of the alga Chlorogonium elongatum as a food source for culturing freshwater hypotrichs. Most freshwater hypotrichs (including Oxytricha nova, O. fallax, and O. trifallax; Euplotes aediculatus and E. eurystomous; and Stylonychia lemnae) can be grown to high density with Chlorogonium as the food organism. A similar regimen can be used to prepare other food sources such as Tetrahymena or bacteria (e.g., Aerobacter aerogenes).
Pristionchus pacificus Genetic Protocols Genetic Protocols for Pristionchus pacificus. Includes: Freezing worms; EMS mutagenesis; Psoralen mutagenesis; Construction of deletion libraries to generate P. pacificus gene knock-outs; Designing primers for the gene of interest; RNAi and morpholino by injection.
Refeeding Marine Euplotids with Bacteria Protocol Feeding euplotids with algae can lead to asynchronous cell starvation and vastly different cell sizes within a culture. Asynchronous starvation also leads to different levels of mating competence. Furthermore, algal pigment remnants can interfere with many applications (e.g., fluorescence microscopy).