Blocking Protocols

Protocols and information related to blocking.

Protocols » Immunohistochemistry Protocols » Blocking Protocols

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Blocking of Unwanted Non-Specific Staining Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Protocol for blocking of unwanted non-specific staining. Includes: Blocking of endogenous enzymes; Blocking of endogenous fluorochromes; Blocking endogenous biotin; Blocking of endogenous Fc blocking; Blocking of crossreactive antigens in the tissue. - [Read Blocking of Unwanted Non-Specific Staining Protocol]

Blocking Solutions Protocols - http://icg.cpmc.columbia.edu/cattoretti/Protocol/main_page_reagents.html#Blocking%20solutions

Protocols for blocking solutions. Includes: Endogenous peroxidase blocking solution (H2O2-Azide); Non-specific antibody background blocking solution: swine serum; Non-specific antibody background blocking solution: MILK; Enzymatic antigen retrieval solution; Unconjugated, biotin- and fluorochrome-conjugated antibody (primary) solutions; Unconjugated, biotin- and fluorochrome-conjugated antibody (secondary) solutions; Enzyme-conjugated secondary antibody solutions.; etc.. - [Read Blocking Solutions Protocols]

Blocking Unwanted Non-Specific Staining Protocol - http://www.ihcworld.com/_protocols/general_IHC/ihc_blocking.htm

Protocol for blocking of unwanted non-specific staining. Includes: Blocking of endogenous enzymes; Blocking of endogenous fluorochromes; Blocking endogenous biotin; Blocking of endogenous Fc blocking; Blocking of cross reactive antigens in the tissue. - [Read Blocking Unwanted Non-Specific Staining Protocol]

Preparing a Cell Block from Cell Lines Cell Suspensions Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/Cell_block.html

Protocol for preparing a cell block from cell lines / cell suspensions. Includes: Live cell preparation; Fixed cell preparation. - [Read Preparing a Cell Block from Cell Lines Cell Suspensions Protocol]