Neurospora Protocols

Protocols and information related to Neurospora.

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Neurospora Protocols Protocol Links Sort by: Hits | Alphabetical

Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae - http://www.fgsc.net/neurosporaprotocols/Cryofixation_NC.pdf

Protocol for cryofixation and freeze substitution for ultrastructural studies of Neurospora crassa hyphae. - [Read Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae]

Generation of Transformable Spheroplasts Protocol - http://www.fgsc.net/fgn39/royer.html

Protocol for generation of transformable spheroplasts from mycelia, macroconidia, microconidia and germinating ascospores of Neurospora crassa. - [Read Generation of Transformable Spheroplasts Protocol]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

How and Where to Obtain Information on Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20and%20where%20to%20obtain%20information.pdf

Information and tips on how and where to obtain information on Neurospora. - [Read How and Where to Obtain Information on Neurospora]

How to Activate Ascospores and Optimize the Recovery of Progeny - http://www.fgsc.net/neurosporaprotocols/How%20to%20activate%20ascospores%20and%20optimize%20the%20recovery%20of%20progeny.pdf

Information and tips on how to activate ascospores and optimize the recovery of progeny. - [Read How to Activate Ascospores and Optimize the Recovery of Progeny]

How to Amplify DNA from Macroconidia - http://www.fgsc.net/neurosporaprotocols/How%20to%20amplify%20DNA%20from%20macroconidia.pdf

Information and tips on how to amplify DNA from macroconidia. - [Read How to Amplify DNA from Macroconidia]

How to Assay Light Responses in Neurospora - http://www.fgsc.net/neurosporaprotocols/lightresponses.pdf

Information and tips on how to assay light responses in Neurospora. - [Read How to Assay Light Responses in Neurospora]

How to Avoid Contamination by Airborne Conidia - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20contamination%20by%20airborne%20conidia.pdf

Information and tips on How to avoid contamination by airborne conidia. - [Read How to Avoid Contamination by Airborne Conidia]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Certify that Neurospora is Nonhazardous - http://www.fgsc.net/neurosporaprotocols/How%20to%20certify%20that%20Neurospora%20final%20.pdf

Information and tips on how to certify that Neurospora is nonhazardous. - [Read How to Certify that Neurospora is Nonhazardous]

How to Choose and Prepare Media - http://www.fgsc.net/neurosporaprotocols/How%20to%20choose%20and%20prepare%20media.pdf

Information and tips on how to choose and prepare media. - [Read How to Choose and Prepare Media]

How to Choose Wild Type Laboratory Strains of N. crassa for Reference and for Special Uses - http://www.fgsc.net/neurosporaprotocols/How%20to%20choose%20wild%20types%20for%20reference%20and%20special%20uses.pdf

Information and tips on how to choose wild type laboratory strains of N. crassa for reference and for special uses. - [Read How to Choose Wild Type Laboratory Strains of N. crassa for Reference and for Special Uses]

How to Color-Code Media - http://www.fgsc.net/neurosporaprotocols/How%20to%20color%20final.pdf

Information on how to color-code media. - [Read How to Color-Code Media]

How to Convert Wild-Type Spreading Growth to Colonial - http://www.fgsc.net/neurosporaprotocols/How%20to%20convert%20wild-type%20spreading%20growth%20to%20colonial.pdf

Information and tips on how to convert wild-type spreading growth to colonial. - [Read How to Convert Wild-Type Spreading Growth to Colonial]

How to Create Gene Knockouts in Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20to%20create%20gene%20knockouts%20in%20Neurospora.pdf

Information and tips on how to create gene knockouts in Neurospora. - [Read How to Create Gene Knockouts in Neurospora]

How to Deal with Mite Infestations and Minimize their Occurrence - http://www.fgsc.net/neurosporaprotocols/How%20to%20deal%20with%20mite%20infections%20and%20to%20minimize%20their%20occurrence%20final.pdf

Information and tips on how to deal with mite infestations and minimize their occurrence. - [Read How to Deal with Mite Infestations and Minimize their Occurrence]

How to Demonstrate Barrage in Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20to%20demonstrate%20barrage%20with%20figure.pdf

Information and tips on how to demonstrate barrage in Neurospora. - [Read How to Demonstrate Barrage in Neurospora]

How to Deposit Materials in the Fungal Genetics Stock Center (FGSC) - http://www.fgsc.net/neurosporaprotocols/How%20to%20deposit%20materials%20final.pdf

Information and tips on how to deposit materials in the Fungal Genetics Stock Center (FGSC). - [Read How to Deposit Materials in the Fungal Genetics Stock Center (FGSC)]

How to Determine Gene Order Using 3-Point Crosses - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20gene%20order%20using%203-point%20crosses%20final.pdf

Information and tips on how to determine gene order using 3-point crosses. - [Read How to Determine Gene Order Using 3-Point Crosses]

How to Determine Mating Type - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20mating%20type%20final.pdf

Information and tips on how to determine mating type. - [Read How to Determine Mating Type]

How to Determine Recessive-Lethal Mutation Rates - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20recessive-leth%20final.pdf

Information and tips on how to determine recessive-lethal mutation rates. - [Read How to Determine Recessive-Lethal Mutation Rates]

How to Determine the Species of a Wild-Collected Isolate - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20the%20species%20of%20a%20wild-collected%20isolate.pdf

Information and tips on how to determine the species of a wild-collected isolate. - [Read How to Determine the Species of a Wild-Collected Isolate]

How to Determine Whether a Gene is Essential for Survival - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20whether%20a%20gene%20is%20essential%20final.pdf

Information and tips on how to determine whether a gene is essential for survival. - [Read How to Determine Whether a Gene is Essential for Survival]

How to Determine Whether a Strain will Undergo Senescence - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20whether%20a%20strain%20will%20undergo%20senescence.pdf

Information and tips on how to determine whether a strain will undergo senescence. - [Read How to Determine Whether a Strain will Undergo Senescence]

How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion - http://www.fgsc.net/neurosporaprotocols/How%20to%20distinguish%20a%20Spore%20kill.pdf

Information and tips on how to distinguish a Spore killer from alternative causes of ascospore abortion. - [Read How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion]