Drosophila Protocols

Protocols and information related to the model organism, Drosophila. Including: Drosophila Dissection; Drosophila Whole-Mount Staining; Drosophila Genetics; Drosophila Media; Drosophila Nucleic Acids.

Protocols » Drosophila Protocols

Drosophila Protocols Categories

Drosophila Culture Media Protocols (13)

Drosophila Dissection Protocols (3)

Drosophila Genetics Protocols (11)

Drosophila Nucleic Acids Protocols (3)

Drosophila Staining Protocols (10)

Drosophila Protocols Protocol Links Sort by: Hits | Alphabetical

Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4526

Enzyme-linked reagents give excellent sensitivity and use a simple light microscope for detection. A range of enzymes is available, but for staining in situ, horseradish peroxidase will suit most needs. Diaminobenzidine (DAB) is one of the most sensitive substrates for horseradish peroxidase. It yields an intense brown product that is insoluble in both water and alcohol. It can be made more sensitive by adding metal salts such as cobalt or nickel to the substrate solution. - [Read Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol]

Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4527

Protocol for antibody addition to Drosophila specimens and detection using fluorochrome-linked reagents. Fluorochrome-linked reagents should be used when high resolution is needed or if two antigens need to be localized simultaneously. Because of the thickness of fly specimens, detection requires access to a confocal microscope. - [Read Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents Protocol]

Drosophila Counting Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#VI.

Protocol for Drosophila counting. - [Read Drosophila Counting Protocol]

Drosophila Embryo Microinjection Protocol - http://vosshall.rockefeller.edu/protocols/DrosophilaEmbryo.pdf

Protocol for Drosophila embryo microinjection. - [Read Drosophila Embryo Microinjection Protocol]

Drosophila Freezing Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#IV.

Protocol for Drosophila freezing. - [Read Drosophila Freezing Protocol]

Drosophila Larval Chemotaxis Assays Protocol - http://vosshall.rockefeller.edu/protocols/LarvalChemotaxisAssay.pdf

Protocol for Drosophila larval chemotaxis assays. - [Read Drosophila Larval Chemotaxis Assays Protocol]

Drosophila Thawing Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#V.

Protocol for Drosophila thawing. - [Read Drosophila Thawing Protocol]

Mechanical Tracing of Protein Function in the Drosophila Ear Protocol - http://www.natureprotocols.com/2006/11/03/mechanical_tracing_of_protein.php

Method relies on the examination of the ear’s mechanics, which is actively modulated by the motility of auditory neurons and reflects the function of mechanosensory proteins these cells comprise [5-7]. Mechanical signatures arising from the motility of the neurons are assayed by measuring the vibrations of the antennal sound receiver in the presence and absence of sound. - [Read Mechanical Tracing of Protein Function in the Drosophila Ear Protocol]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol]