Dnase Footprinting Assay Protocols

Protocols and information related to Dnase Footprinting Assay.

Protocols » DNA Protein Interactions Protocols » Dnase Footprinting Assay Protocols

Dnase Footprinting Assay Protocols Protocol Links Sort by: Hits | Alphabetical

Dnase I Footprinting Protocol - http://www.fhcrc.org/science/labs/tsukiyama/protocols/dnase_i_footprinting.pdf

Protocol for Dnase I footprinting. Includes: Labeling of probe; Synthesis of probe by PCR; Dnase I foot printing. - [Read Dnase I Footprinting Protocol]

DnaseI Footprint Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F2.html

Protocol for DnaseI footprint. - [Read DnaseI Footprint Protocol]

DnaseI Footprinting Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/DNasefootprinting.html

Protocol for DnaseI footprinting assay. - [Read DnaseI Footprinting Assay Protocol]

Footprinting Protocol - http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/F/Footprinting.html

Protocol for footprinting. - [Read Footprinting Protocol]

In Vitro Footprinting Methods Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/5.%20In-vitro%20footprinting.pdf

Protocol for in vitro footprinting methods. Includes: Preparation of labeled probes; Gel purification. - [Read In Vitro Footprinting Methods Protocol]

Mapping Dnase-I-hypersensitive Sites Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3949

Protocol describes how isolated nuclei are incubated with varying amounts of Dnase I. Genomic DNA is then isolated from the nuclei and digested with a restriction enzyme, analyzed by gel electrophoresis, and probed by Southern hybridization. - [Read Mapping Dnase-I-hypersensitive Sites Protocol]

Mapping Protein-binding Sites on DNA by Dnase I Footprinting Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3947

Dnase I is used to fragment a radiolabeled target DNA in the presence and absence of a nuclear extract. A "footprint" is generated when a protein binds to the target and protects a specific segment of DNA from the nucleolytic activity of Dnase I. By comparing the electrophoretic mobility of the Dnase I cleavage products to those of a sequence ladder derived from the same DNA fragment, the position(s) of the DNA sequences recognized by DNA-binding proteins can be determined. - [Read Mapping Protein-binding Sites on DNA by Dnase I Footprinting Protocol]

Maxam Gilbert Sequencing for DnaseI Footprinting Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/M2.html

Quick protocol to give a representative G+A ladder for DnaseI footprint experiments. - [Read Maxam Gilbert Sequencing for DnaseI Footprinting Protocol]