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Topo Cloning Procedure - Details

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Protocols » Listing Details
ID:936
Title: Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html
Description:Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago.
Category:DNA Protocols: TOPO Cloning
Link Owner:
Date Added:July 05, 2006 06:07:06 PM
Number Hits:443
 

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