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Purification of DNA Recovered Anion-exchange Chromatography Protocol - Details

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Protocols » Listing Details
ID:3645
Title: Purification of DNA Recovered Anion-exchange Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/2/pdb.prot4024
Description:Protocol for the purification of DNA recovered from agarose and polyacrylamide gels by anion-exchange chromatography. Fragments of DNA recovered from agarose gels are sometimes poor templates or substrates in subsequent enzymatic reactions.  This problem can be solved by binding the DNA to a positively charged matrix, such as DEAE-Sephadex or DEAE-Sephacel, in buffers of low ionic strength.  After washing the matrix, the DNA is eluted by raising the strength of the buffer.
Category:Chromatography Protocols: Ion Exchange Chromatography Protocols
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Date Added:October 09, 2008 01:50:13 AM
Number Hits:71
 

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