Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol - Details

Link Details 

Protocols » Listing Details
ID:3637
Title: Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4088
Description:Recombinant proteins engineered to have a polyhistidine tail at either the carboxyl or amino terminus can easily be purified in one step by affinity chromatography on a resin carrying chelated nickel ions.  Chromatography can be carried out in column or batch formats.  After unbound proteins are washed away, the target protein is eluted using imidazole, which typically preserves the antigenic and functional features of the protein.
Category:Chromatography Protocols: Affinity Chromatography Protocols
Link Owner:
Date Added:July 13, 2008 02:30:02 PM
Number Hits:139
 

Protocols Tags

  • bacteriophage Protocols
  • banding Protocols
  • based Protocols
  • biology Protocols
  • cancer Protocols
  • cell Protocols
  • cgh Protocols
  • chromosomes Protocols
  • competent Protocols
  • deletions Protocols
  • design Protocols
  • detection Protocols
  • direct Protocols
  • dna Protocols
  • expression Protocols
  • genes Protocols
  • genomic Protocols
  • gfp Protocols
  • has Protocols
  • human Protocols
  • hybridization Protocols
  • immunocytochemical Protocols
  • immunofluorescence Protocols
  • isolation Protocols
  • lambda Protocols
  • large Protocols
  • manual Protocols
  • molecular Protocols
  • mouse Protocols
  • multiplex Protocols
  • optimisation Protocols
  • pcr Protocols
  • plates Protocols
  • pouring Protocols
  • preparation Protocols
  • primer Protocols
  • procedure Protocols
  • process Protocols
  • reaction Protocols
  • review Protocols
  • sequencing Protocols
  • situ Protocols
  • sky Protocols
  • solution Protocols
  • stain Protocols
  • staining Protocols
  • subsets Protocols
  • techniques Protocols
  • that Protocols
  • well Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum