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Protein Ligand Affinity Chromatography Protocol - Details

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Protocols » Listing Details
ID:3635
Title: Protein Ligand Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4203
Description:Protocol describes the coupling of peptides or proteins to Affi-Gel 10 (Bio-Rad).  Affi-Gel 10 is a cross-linked agarose derivatized with N-hydroxysuccinimide (NHS) ester on a 10-atom-long spacer arm.  The NHS ester allows spontaneous coupling of proteins via free amino groups.
Category:Chromatography Protocols: Affinity Chromatography Protocols
Link Owner:
Date Added:July 13, 2008 02:28:37 PM
Number Hits:58
 

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