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Overview of Affinity Purification in Combination with Mass Spectrometry Protocol - Details

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Protocols » Listing Details
ID:3634
Title: Overview of Affinity Purification in Combination with Mass Spectrometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/30/pdb.top3
Description:Protein complexes can be isolated by several different approaches.  For example, a protein can be tagged with an epitope such as Flag or TAP and then overexpressed in a target cell, allowing the interacting proteins to be purified.  Similarly, epitope tags can be homologously recombined into the endogenous locus ("knocked-in"), allowing protein complexes containing the tagged proteins to be isolated at their natural expression level.
Category:Chromatography Protocols: Affinity Chromatography Protocols
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Date Added:July 13, 2008 02:27:56 PM
Number Hits:42
 

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