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Affinity Purification of Interacting Proteins from Cell Lysates Protocol - Details

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Protocols » Listing Details
ID:3632
Title: Affinity Purification of Interacting Proteins from Cell Lysates Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4593
Description:Recombinant protein or a chemically synthesized bioactive fragment is immobilized on resin and used as a probe to capture interacting proteins directly from a cell extract.  Affinity-purified proteins are fractionated by gel electrophoresis and visualized by Coomassie staining.  Proteins that interact specifically are identified by comparing this gel profile to one obtained from cell lysates passed over a control resin lacking the immobilized probe protein.
Category:Chromatography Protocols: Affinity Chromatography Protocols
Link Owner:
Date Added:July 13, 2008 02:26:26 PM
Number Hits:136
 

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