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Purification of Histidine-Tagged Proteins Using IMAC Without Parameter Optimization Protocol - Details

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Protocols » Listing Details
ID:3625
Title: Purification of Histidine-Tagged Proteins Using IMAC Without Parameter Optimization Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4217
Description:Histidine-tagged proteins can be purified on prepacked 1-ml immobilized metal-ion affinity chromatography (IMAC) columns without optimization of the separation conditions.  The method allows fast capture of the target protein, although with a lower purity than can be obtained under optimized conditions.
Category:Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols
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Date Added:July 12, 2008 05:49:21 PM
Number Hits:26
 

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