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Purification of Histidine-Tagged Proteins under Denaturing Conditions Using IMAC Protocol - Details

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Protocols » Listing Details
ID:3624
Title: Purification of Histidine-Tagged Proteins under Denaturing Conditions Using IMAC Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4221
Description:Immobilized metal-ion affinity chromatography (IMAC) is suitable for the purification of proteins under denaturing conditions.  Either guanidine-HCl or urea can be used, although guanidine-HCl is a stronger denaturant than urea.  Proteins that have been adsorbed to the column in the presence of guanidine-binding buffer may be washed with urea-binding buffer and eluted with urea elution buffer.
Category:Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols
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Date Added:July 12, 2008 05:48:32 PM
Number Hits:9
 

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