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Optimization of Imidazole Concentrations for Immobilized Metal-Ion Affinity Chromatography Protocol - Details

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Protocols » Listing Details
ID:3618
Title: Optimization of Imidazole Concentrations for Immobilized Metal-Ion Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/9/pdb.prot4261
Description:Protocol for the optimization of imidazole concentrations for immobilized metal-ion affinity chromatography. Most samples from which histidine-tagged proteins are to be purified also contain endogenous protein contaminants that bind to the immobilized metal-ion affinity chromatography (IMAC) adsorbent.  Usually, these proteins bind more weakly than the histidine-tagged protein.
Category:Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols
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Date Added:July 11, 2008 12:58:17 PM
Number Hits:73
 

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