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Lysis and Protein Extraction from WEHI-231 Cells with TriPure Isolation Reagent Protocol - Details

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Protocols » Listing Details
ID:3617
Title: Lysis and Protein Extraction from WEHI-231 Cells with TriPure Isolation Reagent Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000162.pdf?pid=PP00000162
Description:Protocol is the first step in a three-step process for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by LC-MS/MS.  This procedure is used to prepare protein extracts from WEHI-231 cells.  This preparation method provides total cellular protein samples that are free of contaminating nucleic acids.
Category:Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols
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Date Added:July 11, 2008 12:56:48 PM
Number Hits:14
 

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