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Chelating Sepharose Fast Flow Protocol - Details

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Protocols » Listing Details
ID:3615
Title: Chelating Sepharose Fast Flow Protocol - http://www.bio.indiana.edu/~ybelab/procedures/ChelatingSepharose.pdf
Description:Immobilized metal ion affinity chromatography (IMAC) exploits a molecule’s affinity for chelated metal ions.  The amino acid histidine present in many proteins forms complexes with transition metal ions such as Cu2+, Zn2+, Ni2+ and Fe3+.  Chelating Sepharose™ Fast Flow with a suitable immobilized metal ion will therefore selectively retain proteins with exposed histidine.
Category:Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols
Link Owner:
Date Added:July 11, 2008 12:54:19 PM
Number Hits:11
 

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