Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - Details

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ID:	3515
Title:	Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664
Description:	Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture.
Category:	Neuroscience Protocols: Neuronal Cell Culture Protocols
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Date Added:	June 23, 2008 07:35:19 AM
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