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Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - Details

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Protocols » Listing Details
ID:3426
Title: Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4302
Description:Transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using the submerged method is achieved by placing the gel next to a piece of nitrocellulose filter, submerging this sandwich in a large volume of transfer buffer in a transfer tank, and running current from one side of the transfer tank to another.  The proteins are then eluted by transferring them from the gel onto the filter.
Category:Western Blot Protocols: General Western Blot Methods Protocols
Link Owner:
Date Added:May 10, 2008 02:16:12 AM
Number Hits:83
 

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