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Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - Details

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Protocols » Listing Details
ID:3425
Title: Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4301
Description:The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter.  This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter.
Category:Western Blot Protocols: General Western Blot Methods Protocols
Link Owner:
Date Added:May 10, 2008 02:12:45 AM
Number Hits:110
 

 

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