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Measuring Protein Concentration by Western Analysis Using Enhanced Chemiluminescence Detection - Details

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Protocols » Listing Details
ID:3415
Title: Measuring Protein Concentration by Western Analysis Using Enhanced Chemiluminescence Detection - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4255
Description:Protocol describes, samples containing the target protein are deposited onto a polyvinyldifluoride (PVDF) membrane using a vacuum manifold.  The immobilized protein is exposed to an antibody specific for the target protein, followed by an antibody that reacts with species-specific determinants carried by the primary antibody and is conjugated to horseradish peroxidase (HRP).
Category:Western Blot Protocols: Antigen Detection Methods Protocols
Link Owner:
Date Added:May 10, 2008 01:48:43 AM
Number Hits:9
 

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