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Competitive RT-PCR: Preparation of Competitor RNA Protocol - Details

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Protocols » Listing Details
ID:3407
Title: Competitive RT-PCR: Preparation of Competitor RNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4110
Description:The first step in competitive RT-PCR is the synthesis and purification of the synthetic competitor.  This is an RNA molecule designed to be reverse-transcribed and PCR-amplified with the same efficiency as the endogenous transcript of interest.  Once the competitor molecule has been prepared, as described in this protocol, competitive PCR can be carried out.
Category:PCR Protocols: Reverse Transcriptase RT-PCR Protocols
Link Owner:
Date Added:April 21, 2008 02:21:16 AM
Number Hits:25
 

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