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Competitive RT-PCR: Estimation of Copy Number Protocol - Details

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Protocols » Listing Details
ID:3406
Title: Competitive RT-PCR: Estimation of Copy Number Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4111
Description:In this protocol, sample and competitor RNAs are reverse transcribed (separately) in a pilot experiment.  A constant amount of sample RT product is then combined with a 2-logserial dilution of competitor RT product for PCR. Procedure provides an approximate copy number for the sample, which is then fine-tuned by repeating the experiment with a series of twofold dilutions of competitor.  The experiment includes controls for sample-to-sample variations in RT efficiency.
Category:PCR Protocols: Reverse Transcriptase RT-PCR Protocols
Link Owner:
Date Added:April 21, 2008 02:19:58 AM
Number Hits:37
 

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