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Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium - Details

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Protocols » Listing Details
ID:3405
Title: Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4115
Description:Protocol uses a single thermostable RNA polymerase to perform high-specificity RT-PCR.  A high-temperature RT reaction is followed by PCR amplification of the cDNA using a single thermostable poymerase, the GeneAmp AccRT RNA PCR enzyme from Applied Biosystems.  The high temperature of the RT reaction enhances the specificity of primer binding and also reduces secondary structure in the template, thereby increasing the efficiency of polymerization.
Category:PCR Protocols: Reverse Transcriptase RT-PCR Protocols
Link Owner:
Date Added:April 21, 2008 02:18:27 AM
Number Hits:109
 

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