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Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization Protocol - Details

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Protocols » Listing Details
ID:3400
Title: Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization Protocol - https://commerce.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeId=3DD21299A00419DEBAFDBC78E47CBE79
Description:Method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to optimize for specificity, sensitivity, and yield.  The first step of PCR simply entails mixing template DNA, two appropriate oligonucleotide primers, Taq or other thermostable DNA polymerases, deoxyribonucleoside triphosphates (dNTPs), and a buffer.
Category:PCR Protocols: PCR Optimization
Link Owner:
Date Added:April 21, 2008 02:02:39 AM
Number Hits:910
 

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