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PCR Amplification of Highly GC-Rich Regions Protocol - Details

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Protocols » Listing Details
ID:3398
Title: PCR Amplification of Highly GC-Rich Regions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4093
Description:Procedure details the establishment of an amplification procedure for GC-rich sequences.  The DNA fragments of interest are amplified in the presence of either 5% DMSO, 1 M betaine, 2 M betaine, 1 M betaine, and 5% DMSO; 2 M betaine and 5% DMSO; 0.4 M tetramethylene sulfone; or without any of the enhancers.
Category:PCR Protocols: PCR Optimization
Link Owner:
Date Added:April 21, 2008 01:56:24 AM
Number Hits:362
 

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