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Cap-Switching RACE Protocol - Details

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Protocols » Listing Details
ID:3385
Title: Cap-Switching RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4133
Description:This method, for the selective amplification of full-length cDNA ends, involves the addition of an adapter during reverse transcription.  This method takes advantage of the propensity of Moloney murine leukemia virus reverse transcriptase (MMLV RT) to append two to four cytosines to the 3'-end of newly synthesized cDNA strands.  The additional residues are added when the enzyme reaches the 5'-cap structure at the end of the mRNA template.
Category:PCR Protocols: cDNA Synthesis Protocols
Link Owner:
Date Added:April 20, 2008 12:53:44 PM
Number Hits:33
 

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