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5'-End cDNA Amplification Using Classic RACE Protocol - Details

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Protocols » Listing Details
ID:3382
Title: 5'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4131
Description:To generate "5'-end" partial cDNA clones using classic RACE, the first-strand products are generated by reverse transcription (primer extension) from a known gene-specific primer (GSP-RT).  Then, a poly(A) tail is appended using terminal deoxynucleotidyltransferase (Tdt) and dATP.  Amplification is carried out using three primers.
Category:PCR Protocols: cDNA Synthesis Protocols
Link Owner:
Date Added:April 20, 2008 12:49:59 PM
Number Hits:156
 

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