5'-End cDNA Amplification Using Classic RACE Protocol - Details

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ID:	3382
Title:	5'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4131
Description:	To generate "5'-end" partial cDNA clones using classic RACE, the first-strand products are generated by reverse transcription (primer extension) from a known gene-specific primer (GSP-RT). Then, a poly(A) tail is appended using terminal deoxynucleotidyltransferase (Tdt) and dATP. Amplification is carried out using three primers.
Category:	PCR Protocols: cDNA Synthesis Protocols
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Date Added:	April 20, 2008 12:49:59 PM
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