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3'-End cDNA Amplification Using Classic RACE Protocol - Details

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Protocols » Listing Details
ID:3381
Title: 3'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4130
Description:To generate "3'-end" partial cDNA clones, mRNA is reverse-transcribed using a "hybrid" primer (Qtotal, QT) that consists of two mixed bases (GATC/GAC followed by [T]17) and a unique 35-base oligonucleotide sequence (QI-QO).  Amplification is then performed using a primer containing part of this sequence (Qouter, Qo) (which now binds to each cDNA at its 3'-end) and a primer derived from the gene of interest, GSP1 (gene-specific primer 1).
Category:PCR Protocols: cDNA Synthesis Protocols
Link Owner:
Date Added:April 20, 2008 12:48:48 PM
Number Hits:187
 

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