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Real-Time PCR Protocol - Details

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Protocols » Listing Details
ID:3377
Title: Real-Time PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4112
Description:Protocol uses FAM-(6-carboxy-fluorescein) or JOE-(6-carboxy-4', 5' -dichloro-2',7' -dimethoxy-fluorescein) labeled LUX (Light Upon eXtension) primers, which can quantify 100 or fewer copies of the target DNA in a background of nonspecific templates, over a broad dynamic range of less than 100-107 copies.  It uses uracil deglycosylase (UDG) to minimize the risk of carryover contamination, and includes a melting curve analysis of the product.
Category:PCR Protocols: Real-Time PCR Protocols
Link Owner:
Date Added:April 19, 2008 05:04:30 AM
Number Hits:38
 

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