Molecular Cloning of PCR Products Protocol- https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=3DC8DA9CD690052F2234D07559CD9BFE&objectid=6676A195E9797060CC491B4B58ECC2E1
Description:
The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This protocol describes the strategies for generating and manipulating suitable ends on the PCR fragments.