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Directional Cloning of PCR Products Protocol - Details

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Protocols » Listing Details
ID:3364
Title: Directional Cloning of PCR Products Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4140
Description:Protocol is for directional blunt-end cloning of DNA fragments.  The target DNA is PCR-amplified, 3'-extensions are polished with Pfu DNA polymerase, and the amplicon is ligated to a blunt-ended plasmid DNA.  The products of the ligation reaction are used to transform competent Escherichia coli.  A restriction enzyme is added to the ligation reaction to relinearize any self-religating vector DNA.
Category:PCR Protocols: PCR Cloning Protocols
Link Owner:
Date Added:April 12, 2008 08:07:02 AM
Number Hits:121
 

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