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Blunt-end Cloning of PCR Products Protocol - Details

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Protocols » Listing Details
ID:3361
Title: Blunt-end Cloning of PCR Products Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3830
Description:Protocol for blunt-end cloning of PCR products. Incubation of a blunt-end ligation reaction in the presence of an excess amount of an appropriate restriction enzyme can dramatically increase the yield of recombinant plasmids.  The role of the restriction enzyme is to cleave circular and linear concatemers at restriction sites that are re-formed when linear, blunt-ended plasmid molecules ligate to themselves.  I
Category:PCR Protocols: PCR Cloning Protocols
Link Owner:
Date Added:April 12, 2008 07:29:24 AM
Number Hits:310
 

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