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Bidirectional Cloning of PCR Products Protocol - Details

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Protocols » Listing Details
ID:3360
Title: Bidirectional Cloning of PCR Products Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4139
Description:Protocol is for bidirectional, blunt-end cloning of DNA fragments.  The target DNA is PCR amplified and 3'-extensions are polished with Pfu DNA polymerase.  The amplicon is ligated to a blunt-ended plasmid DNA, and the products of the ligation reaction are used to transform competent Escherichia coli.  A restriction enzyme is added to the ligation reaction to relinearize any self-religating vector DNA.
Category:PCR Protocols: PCR Cloning Protocols
Link Owner:
Date Added:April 12, 2008 07:28:17 AM
Number Hits:166
 

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