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Colony PCR Protocol II - Details

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Protocols » Listing Details
ID:3347
Title: Colony PCR Protocol II - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4141
Description:Protocol describes the use of PCR to screen for bacteria that carry recombinant plasmids.  The PCR can be carried out using the same primers as for amplification of the cloned insert.  To determine the orientation of the insert, a third, insert-specific primer that is asymmetrically distanced from the clonal insertion site can be used.
Category:PCR Protocols: Colony PCR
Link Owner:
Date Added:April 12, 2008 07:03:50 AM
Number Hits:143
 

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