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A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissue - Details

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Protocols » Listing Details
ID:3333
Title: A Single-step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissue - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4056
Description:Protocol for a single-step method for the simultaneous preparation of DNA, RNA, and protein from cells and tissues. The yield of total RNA depends on the tissue or cell source, but it is generally in the range of 4-7 µg/mg starting tissue or 5-10 µg/106 cells.  IMPORTANT: Prepare all reagents used in this protocol with Diethyl pyrocarbonate (DEPC)-treated H2O.
Category:Nucleic Acid Purification Protocols
Link Owner:
Date Added:April 10, 2008 09:23:15 PM
Number Hits:193
 

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