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Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - Details

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Protocols » Listing Details
ID:3309
Title: Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038
Description:Simple protocol is used to extract DNA from small numbers of cultured cells and from fragments of soft or bony tissues.  The method is used chiefly to genotype transgenic and knockout mice.  Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs.
Category:Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols
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Date Added:April 10, 2008 04:20:50 AM
Number Hits:47
 

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