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Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes Protocol - Details

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Protocols » Listing Details
ID:3269
Title: Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4044
Description:Protocol for southern hybridization of radiolabeled probes to nucleic acids immobilized on membranes. Protocol describes how to carry out Southern hybridizations at high stringency in phosphate-SDS buffers.  Although a wide variety of formats are available, most Southern hybridizations are carried out in heat-sealable bags, roller bottles, or plastic boxes.
Category:Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols
Link Owner:
Date Added:April 08, 2008 01:02:08 PM
Number Hits:32
 

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