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Southern Blotting: Capillary Transfer of DNA to Membranes Protocol - Details

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Protocols » Listing Details
ID:3267
Title: Southern Blotting: Capillary Transfer of DNA to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4040
Description:Protocol describes the first stages of Southern blotting: digestion of genomic DNA with one or more restriction enzymes, separation of the resulting fragments by electrophoresis through an agarose gel, and transfer of the denatured fragments to a membrane by downward capillary transfer.
Category:Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols
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Date Added:April 08, 2008 12:59:23 PM
Number Hits:63
 

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